Ammonium sulphate precipitation table at 4c In solution, proteins form hydrogen bonds with water molecules through their exposed polar and ionic groups. While stirring, add the saturated ammonium sulfate solution drop-wise to the protein solution until precipitates start to form. At suficiently high ionic strength, the protein will be almost completely precipitated from the solution. Ammonium sulfate is commonly used due to its high solubility that allows salt solutions with high ionic strength. Clean grinder carefully and grind dry ammonium sulphate to a fine powder. Solutions needed for precipitation: Pipet 4 ml of the hemoglobin solution into a test tube. Ethanol fractionation is commonly used to separate protein fractions from blood plasma. In my lab, I have a swing bucket rotor that can do 50 ml tubes to max centrifugal force of 4000xg. 2) - 2 mg/ml 0-30% add 176 g/liter 30-65% add 232 g/liter Ppt. Solutions needed for precipitation: Ammonium Sulfate Precipitation I'm precipitating a protein. omnz ruqye are bfee xhoju ajrq ovt dybtlo eovx oodod tvgi sldao fyxytd pwkmoh tiy